# Sublingual nitric oxide plus whole-body vibration raised blood amyloid-β in 29 healthy adults

- Published: 2026-11-10 11:18 UTC
- Section: Life & Health › Neurotechnology
- Tags: brain clearance, amyloid-β in blood, sublingual nitric oxide, whole-body vibration, Piezo1 stimulation, Alzheimer's disease, cerebrospinal fluid efflux, magnetic resonance encephalography, University of Oulu, healthy volunteers
- URL: https://realtech.media/news/rt-33b13c9cceab2382da7ae9d4_sublingual_nitric_oxide/

**In healthy volunteers, the combination changed brain fluid dynamics on MRI and raised blood levels of brain-derived proteins, including Aβ40, Aβ42 and GFAP.**

![Sublingual nitric oxide plus whole-body vibration raised blood amyloid-β in 29 healthy adults](https://realtech.media/media/2026/11/d46dda782e637ded.png)
*science.mail.ru*

Researchers from the University of Oulu (Finland) tested a combination of sublingual nitric oxide (NO) and Piezo1-targeted whole-body vibration (WBVp) in 29 healthy adults, according to a study published on nature.com (EudraCT 2021-000625-27) [1]. They used multimodal neuroimaging and blood biomarker analysis to check whether the intervention shifts soluble brain-derived proteins toward the circulation [1].

## Why brain protein clearance matters

The authors propose that an age-related impairment of NO-mediated vasodilation and of Piezo1-dependent vasoconstriction may precede the accumulation of soluble proteins such as amyloid-β and the disruption of brain fluid homeostasis in Alzheimer's disease [1]. In rodents, NO donors increased the movement of tracers from the cranial cavity to the blood, mainly via cervical lymph nodes. Mechanical stimulation of Piezo1 increased meningeal lymphatic drainage [1].

Earlier work showed that low-frequency (≤40 Hz) whole-body vibration improved cognition and motor function in humans and reduced amyloid burden in animal models [1]. On this basis the team hypothesized that combining both effects would raise blood concentrations of brain-derived proteins and alter T2-weighted dark-fluid MRI signal and brain pulsation dynamics [1].

## What was measured and found

The protocol combined T2-weighted dark-fluid MRI, ultrafast magnetic resonance encephalography (MREG) of brain pulsations, and blood measurement of Aβ40, Aβ42 and glial fibrillary acidic protein (GFAP) [1]. The combined intervention was associated with correlating changes in brain fluid dynamics and with higher blood levels of Aβ40, Aβ42 and GFAP [1]. These effects increased with age and with the magnitude of NO-induced hypotension [1].

The interventions were well tolerated under controlled experimental conditions, and the pre-specified primary endpoints were met [1]. The authors describe the findings as consistent with a transient redistribution of brain-derived proteins toward the circulation [1]. According to the publication's retelling, MRI showed changes in fluid movement mainly near large veins, and the researchers reported evidence that the proteins enter large veins in the posterior part of the brain.

The method has so far been tested only in healthy adults, so it is not known whether it would help people with memory impairment. As the next step, the researchers plan to study possible benefit in patients with early signs of such impairment.

## Sources

1. [nature.com](https://www.nature.com/articles/s41467-026-78894-y)
2. [science.mail.ru](https://science.mail.ru/news/60181-vibraciya-i-preparat-protiv-demencii)

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Source: RealTech, realtech.media — https://realtech.media/news/rt-33b13c9cceab2382da7ae9d4_sublingual_nitric_oxide/